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TNF-beta (human) ELISA Kit

Catalog#: K4780-100
Size: 100 assays
Price: 10425 (RMB)
 


Assay Kit Name:
TNF-beta (human) ELISA Kit

Kit Summary:
• Detection method - Absorbance (450 nm)
• Species reactivity - human
• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results

Sample Type:
• Serum & plasma (human TNF-beta concentration is pretty low in normal serum & plasma, it may not be detected in this assay)
• Cell culture supernatants
• Urine

Features & Benefits:
• Easy, convenient and time-saving method to assay TNF-beta.
• The minimum detectable concentration of TNF-beta is less than 60 pg/ml.
• This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL- 12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TPO, VEGF.

Kit Components:
• TNF-beta Microplate (Item A) coated with anti-human TNF-beta, 96 wells
• Wash Buffer Concentrate (20x) (Item B)
• Standards (Item C) (recombinant human TNF-beta)
• Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative)
• Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated)
• Detection Antibody TNF-beta (Item F), biotinylated anti-human TNF-beta (each vial enough for half plate)
• HRP-Streptavidin Concentrate (Item G), 700x concentrated
• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution
• Stop Solution (Item I), 0.2 M sulfuric acid

Description:
BioVision’s Human TNF-beta (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-beta. This assay employs an antibody specific for human TNF-beta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNF-beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL- 12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TPO, VEGF. The minimum detectable dose of TNF-beta is typically less than 60 pg/ml. Detection Range: 60 pg/ml – 18,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

 
 
 
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