Triglyceride Quantification Colorimetric/Fluorometric Kit,NAD/NADH Quantitation Colorimetric Kit,Pyruvate Colorimetric/Fluorometric Assay Kit,Lactate Colorimetric/Fluorometric Assay Kit
 
 
 
 
您當前的位置:首頁 > 2013 New Products > TNF-alpha (human) ELISA Kit
 
TNF-alpha (human) ELISA Kit

Catalog#: K4779-100
Size: 100 assays
Price: 10425 (RMB)
 


Assay Kit Name:
TNF-alpha (human) ELISA Kit

Kit Summary:
• Detection method - Absorbance (450 nm)
• Species reactivity - human
• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results

Sample Type:
• Serum & plasma (human TNF-alpha concentration is pretty low in normal serum & plasma, it may not be detected in this assay)
• Cell culture supernatants
• Urine

Features & Benefits:
• Easy, convenient and time-saving method to assay TNF-alpha.
• The minimum detectable concentration of TNF-alpha is less than 30 pg/ml.
• This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO- alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ1, TGFβ2, TGFβ3, VEGF.

Kit Components:
• TNF-alpha Microplate (Item A) coated with anti-human TNF-alpha, 96 wells
• Wash Buffer Concentrate (20x) (Item B)
• Standards (Item C) (recombinant human TNF-alpha)
• Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative)
• Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated)
• Detection Antibody TNF-alpha (Item F), biotinylated anti-human TNF-alpha (each vial enough for half plate)
• HRP-Streptavidin Concentrate (Item G), 600x concentrated
• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution
• Stop Solution (Item I), 0.2 M sulfuric acid

Description:
BioVision’s Human TNF-alpha (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-alpha. This assay employs an antibody specific for human TNF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO- alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ1, TGFβ2, TGFβ3, VEGF. The minimum detectable dose of TNF-alpha is typically less than 30 pg/ml. Detection Range: 30 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

 
 
 
電話:021-64089408  傳真:021-64088498   上海維晟生物科技有限公司 版權所有
美女任你摸-美女禁区-美女销魂