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Leptin (human) ELISA Kit

Catalog#: K4777-100
Size: 100 assays
Price: 10425 (RMB)
 


Assay Kit Name:
Leptin (human) ELISA Kit

Kit Summary:
• Detection method - Absorbance (450 nm)
• Species reactivity - human
• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results

Sample Type:
• Serum & plasma
• Cell culture supernatants
• Urine

Features & Benefits:
• Easy, convenient and time-saving method to assay leptin.
• The minimum detectable concentration of leptin is less than 2 pg/ml.
• This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Kit Components:
• Leptin Microplate (Item A) coated with anti-human Leptin, 96 wells
• Wash Buffer Concentrate (20x) (Item B)
• Standards (Item C) (recombinant human Leptin)
• Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative)
• Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated)
• Detection Antibody Leptin (Item F), biotinylated anti-human Leptin (each vial enough for half plate)
• HRP-Streptavidin Concentrate (Item G), 160x concentrated
• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution
• Stop Solution (Item I), 0.2 M sulfuric acid

Description:
BioVision’s Human Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Leptin. This assay employs an antibody specific for human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of Leptin is typically less than 2 pg/ml. Detection Range: 2 pg/ml - 400 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

 
 
 
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