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GM-CSF (human) ELISA Kit

Catalog#: K4772-100
Size: 100 assays
Price: 10425 (RMB)
 


Assay Kit Name:
GM-CSF (human) ELISA Kit

Kit Summary:
• Detection method - Absorbance (450 nm)
• Species reactivity - human
• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results

Sample Type:
• Serum & plasma
• Cell culture supernatants
• Urine

Features & Benefits:
• Easy, convenient and time-saving method to assay GM-CSF.
• The minimum detectable concentration of GM-CSF is less than 2 pg/ml.
• This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

Kit Components:
• GM-CSF Microplate (Item A) coated with anti-human GM-CSF, 96 wells
• Wash Buffer Concentrate (20x) (Item B)
• Standards (Item C) (recombinant human GM-CSF)
• Assay Diluent (Item E) for Standard/Sample (serum/plasma/ cell culture medium/Urine) diluent (5x con.)
• Detection Antibody GM-CSF (Item F), biotinylated anti-human GM-CSF (each vial enough for half plate)
• HRP-Streptavidin Concentrate (Item G), 200x concentrated
• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution
• Stop Solution (Item I), 0.2 M sulfuric acid

Description:
BioVision’s human GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GM-CSF. This assay employs an antibody specific for human GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. The minimum detectable dose of GM-CSF is typically less than 2 pg/ml. Detection Range: 2 pg/ml - 500 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

 
 
 
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