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DPP IV (Mouse) ELISA Kit

Catalog#: K4770-100
Size: 100 assays
Price: 10425 (RMB)
 


Assay Kit Name:
DPP IV (Mouse) ELISA Kit

Kit Summary:
• Detection method - Absorbance (450 nm)
• Species reactivity - Mouse
• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results

Sample Type:
• Serum & plasma
• Cell culture supernatants

Features & Benefits:
• Easy, convenient and time-saving method to assay DPP 4.
• The minimum detectable concentration of DPP 4 is less than 0.45 ng/ml.
• This ELISA kit shows no cross-reactivity with the following cytokines tested:Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

Kit Components:
• DPP IV Microplate (Item A) coated with anti-mouse DPP IV, 96 wells
• Wash Buffer Concentrate (20x) (Item B)
• Standards (Item C) (recombinant mouse DPP IV)
• Assay Diluent C (Item L) for Standard/Sample (serum/plasma/ cell culture supernatant) diluent
• Assay Diluent B (Item E) for detection antibody and HRP- Streptavidin diluent (5x concentrated)
• Detection Antibody DPP IV (Item F), biotinylated anti-mouse DPP IV (each vial enough for half plate)
• HRP-Streptavidin Concentrate (Item G), 200x concentrated
• TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution
• Stop Solution (Item I), 0.2 M sulfuric acid

Description:
BioVision’s Mouse DPP IV ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DPP IV. This assay employs an antibody specific for DPP IV coated on a 96-well plate. Standards and samples are pipetted into the wells and DPP IV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DPP IV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPP IV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of DPP IV is typically less than 0.45 ng/ml. Detection Range: 0.45 ng/ml - 100 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

 
 
 
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