TM HMGB1 /HMG1, human recombinant>
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Human CellExpTM HMGB1 /HMG1, human recombinant

Catalog#: 7240-10
Size: 10 μg
Price: 2025 (RMB)

Product Name: Human CellExp™ HMGB1 /HMG1, human recombinant

Alternate Name/Synonyms: HMGB1, HMG1, HMG3, SBP-1

Gene Symbol: HMGB1

Accession #: P09429

Gene ID: 3146

Source: HEK 293 cells

Appearance: Lyophilized powder

Physical Form Description: Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Molecular Weight: The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.7 kDa. The predicted N-terminus is Met1. DTT-reduced Protein migrates as 28 kDa and 32 kDa due to different glycosylation.

Purity by SDS-PAGE: ≥95%

Purity by HPLC: N/A

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Biological Activity: Measured by its binding ability in a functional ELISA Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.22 μg/ml.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: High-mobility group protein B1 (HMGB1), also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.

Amino Acid Sequence: N/A

Handling: Centrifuge the vial prior to opening

USAGE: For Research Use Only! Not For Use in Humans.

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