Product Name: Human CellExp™ ADIPOQ /Adiponectin /ACRP30, human recombinant
Alternate Name/Synonyms: ADIPOQ, Adiponectin, ACDC, ACRP30, APM1, GBP28
Gene Symbol: ADIPOQ
Accession #: Q15848
Gene ID: 9370
Source: HEK 293 cells
Appearance: Lyophilized powder
Physical Form Description: Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight: The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.4 kDa. The predicted N-terminus is Glu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.
Purity by SDS-PAGE: ≥92%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Measured by its ability to induce TIMP-1 secretion by mouse macrophages. The ED50 for this effect is typically 2 - 10 µg/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Adiponectin also referred to as GBP-28, APM1, ADIPOQ, ACDC and Acrp30, is a secreted protein which contains one C1q domain (commonly called the globular domain) and one collagen-like domain. Adiponectin / ADIPOQ is synthesized exclusively by adipocytes and secreted into plasma. ADIPOQ is important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Adiponectin antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Acrp30 Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway and may play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.