roduct Name: Human CellExp™ IL-27, human recombinant
Alternate Name/Synonyms: p28, IL30, IL-27, IL-27A, IL27p28, Interleukin-27, EBI3
Gene Symbol: IL-27
Accession #: Q8NEV9
Gene ID: 246778
Source: HEK 293 cells
Appearance: Lyophilized powder
Physical Form Description: Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight: The rhIL27 protein complex is composed of IL27B/EBI-3 subunit and the NT truncated IL27A / p28 subunit linked by a polypeptide linker (GSGSSRGGSGSGGSGGGGSKL) with 6 × His tag at the C-terminus. It has a calculated MW of 50.3 kDa. The predicted N-terminus is Arg 21. DTT-reduced Protein migrates as 60 kDa due to glycosylation.
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhIL-27 at 20 μg/ml (100 μl/well) can bind human IL-27Ra with a linear ranger of 0.032 - 1.2 μg/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. IL-27 plays an important function in regulating the activity of B and T lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell surface receptor complex composed of two proteins known as IL27R and gp130. IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity and anti-angiogenic activity.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.