TM SHH, human recombinant>
Triglyceride Quantification Colorimetric/Fluorometric Kit,NAD/NADH Quantitation Colorimetric Kit,Pyruvate Colorimetric/Fluorometric Assay Kit,Lactate Colorimetric/Fluorometric Assay Kit
 
 
 
 
您當前的位置:首頁 > 2013 New Products > Human CellExpTM SHH, human recombinant
 
Human CellExpTM SHH, human recombinant

Catalog#: 7223-10
Size: 10 μg
Price: 2025 (RMB)
 


Product Name: Human CellExp™ SHH, human recombinant

Alternate Name/Synonyms: SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS

Gene Symbol: SHH

Accession #: Q15465

Gene ID: 6469

Source: HEK 293 cells

Appearance: Lyophilized powder

Physical Form Description: Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Molecular Weight: This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 20.4 kDa. The predicted N-terminus is Cys 24. DTT-reduced protein migrates as 22 kDa.

Purity by SDS-PAGE: ≥95%

Purity by HPLC: N/A

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Biological Activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 μg/mL.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: Sonic hedgehog protein (SHH) also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an auto-proteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

Amino Acid Sequence: N/A

Handling: Centrifuge the vial prior to opening

USAGE: For Research Use Only! Not For Use in Humans.

 
 
 
電話:021-64089408  傳真:021-64088498   上海維晟生物科技有限公司 版權所有
美女任你摸-美女禁区-美女销魂