Product Name: EDEM2 Antibody
Antibody Target: EDEM2
Alternative Name: ER degradation-enhancing alpha-mannosidase-like protein 2
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide C-terminal
Gene ID: 55741
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified antibody
Species Reactivity: Human, mouse, rat
Application: Western blot
Positive Control: Western Blot: Rat kidney lysate
Application & Usage: Western blot: 1:200
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Proteins expressed in the endoplasmic reticulum (ER) are tightly regulated by a variety of quality control mechanisms. Terminally misfolded proteins in the ER are retrotranslocated to the cytoplasm and degraded by proteasomes through a mechanism known as ER-associated degradation (ERAD). EDEM2 (ER degradation-enhancing alpha-mannosidase-like 2) is a 578 amino acid secreted protein that, in conjunction with other EDEM proteins (namely EDEM and EDEM3), is involved in the ERAD pathway of protein degradation. EDEM2, a member of the glycosyl hydrolase 47 family, contains a mannosidase homology domain, an N-terminal cleavable signal sequence and a C-terminal extension that is required for both ER retention and the proper function of EDEM2. Human EDEM2 shares 93% sequence identity with its mouse homolog, suggesting a conserved role between species. Two isoforms of EDEM2 exist due to alternative splicing events.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.